Part:BBa_K1021000

crtE

This is the coding sequence of crtE, which encodes geranylgeranyl pyrophosphate synthase, an enzyme that converts farnesyl pyrophosphate (FPP) to geranylgeranyl pyrophosphate synthase (GGPP) as part of the carotenoid biosynthetic pathway.

Adapted from KEGG reference pathways

crtE was originally from the 2011 Edinburgh team's biobrick (BBa_K523022) that is a assembly of carotenoid genes crtE, crtB, and crtI, all originating from Pantoea ananatis. crtE encodes geranylgeranyl pyrophosphate synthase, part of the carotenoid biosynthesis pathway, which converts farnesyl pyrophosphate to geranylgeranyl pyrophosphate (Misawa et al., 1990).

1. Misawa, N., Nakagawa, N., Kobayashi, K., Yamano, S., Nakamura, K., and Harashima, K. 1990. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. Journal of Bacteriology 172, 6704-6712.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 724
  
• 1000
  COMPATIBLE WITH RFC[1000]